Isolation and 2-D-DIGE proteomic analysis of intracellular and extracellular forms of Listeria monocytogenes

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Isolation and 2-D-DIGE proteomic analysis of intracellular and extracellular forms of Listeria monocytogenes.

The pathogenicity of Listeria monocytogenes is related to its ability of invading and multiplying in eukaryotic cells. Its main virulence factors are now well characterized, but limited proteomic data is available concerning its adaptation to the intracellular environment. In this study, L. monocytogenes EGD (serotype 1/2a) grown in human THP-1 monocytes (24 h) were successfully separated from ...

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ژورنال

عنوان ژورنال: PROTEOMICS

سال: 2009

ISSN: 1615-9853,1615-9861

DOI: 10.1002/pmic.200900503